Coding

Part:BBa_K1181000:Design

Designed by: Sebastian Roy, Maja Rennig   Group: iGEM13_Frankfurt   (2013-09-25)

Kaurenoic Acid Hydroxylase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 293
    Illegal BglII site found at 1095
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 4
    Illegal BsaI site found at 798


Design Notes

This gene is intended to be used with the bifunctional ent-Kaurene oxidase and ent-kaurene synthase. Together they are responsible for the pathway from geranylgeranyl pyrophosphate to steviol.


Source

DNA was synthesized by GeneScript. The gene is from the plant stevia rebaudiana.

References

The sequence coding for this enzyme has been patented by Jim Brandle under [http://www.google.com/patents/CA2580429A1?cl=en EP 1897951]:

[http://www.sciencedirect.com/science/article/pii/S0003986196903367 Hydroxylation of ent-kaurenoic acid to steviol in Stevia rebaudiana Bertoni--purification and partial characterization of the enzyme.] Kim KK, Sawa Y, Shibata H.